BioAssistant was written to improve on Probe Assistant, CSL s first attempt at a tool designed to assist PCR chemists create DNA Probes. With BioAssistant, you can do all the things you could do with Probe Assistant (such as determine probe Melting Points (Tm), Molecular Weights, Hybridization information and other useful tidbits), but with a much cleaner and easier-to-use interface. BioAssistant also extends on Probe Assistant s feature set by introducing several useful tools, such as a Master Mix Calculator.